Haynes Lab Members to Present Research at Fall 2017 EBRC Retreat

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ebrc_logo3Haynes lab members Alyssa Henning, Ben Nyer, and Dr. Karmella Haynes will be presenting research at a 2017 Fall Retreat organized by the Engineering Biology Research Consortium (EBRC). The retreat will take place at Georgia Tech in Atlanta, GA September 22 – 23, 2017. EBRC members include some of the most prestigious labs in synthetic biology. Attendees will come from academic, industry, and government institutions from across the country.

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News: Haynes lab grad student Alyssa Henning gets creative with science outreach

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Alyssa Henning at Saboten Con, September 2017.

Haynes lab PhD student Alyssa Henning (Biological Design) recently participated in local Comic Con and cultural events in Phoenix. The first event was Phoenix Comic Con on May 25 – 28 where she was invited to talk about real-life science as a panelist. At a Japanese pop culture event called Saboten Con (September 4), Alyssa and the Sun Devil Taiko club (of which she is an officer) won an award for their musical performance.

Sun Devil Taiko also performed at Culture Fest on Tuesday, August 15th. You can watch a recorded performance of Ogi Matsuri and Taiko Bayashi online.

 

 

 


Related articles:

Research – bioRxiv Pre-print – Activation of tumor suppressor genes in breast cancer cells by a synthetic chromatin effector

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Activation of tumor suppressor genes in breast cancer cells by a synthetic chromatin effector
Olney KC, Nyer DB, Wilson Sayres MA, Haynes KA. (2017) bioRxiv. http://www.biorxiv.org/content/early/2017/09/07/186056

Certain types of breast cancer can be difficult to treat because breast cancer cells in different patients are not completely identical. Here, we measured the expression levels of genes in drug responsive and non-responsive (triple-negative) lab-grown breast cancer cells. In agreement with findings from other research groups, we observed that certain groups of genes are commonly or differentially expressed. Importantly, a large group of genes is silenced in breast cancer cells compared to less cancerous cells. In cancers, certain overactive proteins silence genes by inducing tight chromatin packing. We used a synthetic fusion protein called PcTF to bind and disrupt cancer-associated gene silencing. Dozens of genes, including fifteen different anti-cancer genes became activated in all of the cancer cell types, including the triple-negative cells. PcTF has the potential to act as a powerful therapeutic protein (biologic) that activates multiple anti-cancer genes at once.

News: “Soft” Side of Bioengineering Poised to Make Big Impacts

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ASU Full Circle recently highlighted the emerging Molecular, Tissue, and Cellular Bioengineering (MCTB) community at ASU and the second annual MCTB Symposium, co-chaired by Karmella Haynes.

J. Kullman, 07.27.17, ASU Full Circle: There are more technically precise descriptions of what’s at the core of a growing trend broadening the horizons of biomedical engineering than “the soft, squishy side of bioengineering.” But Karmella Haynes and Kaushal Rege still like the way that sums up what they and about 20 other Arizona State University faculty members are increasingly focusing on in their research and teaching.”

Read more at ASU Full Circle.

Review – NAR – Molecular structures guide the engineering of chromatin

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Molecular structures guide the engineering of chromatin
Tekel SJ and Haynes KA (2017) Nucleic Acids Res. https://doi.org/10.1093/nar/gkx531

Specialized proteins within the nuclei of human and other eukaryotic cells wrap DNA into a structure called chromatin. For decades, scientists have used biochemistry, genetics, and comparative evolutionary biology to understand the specific interactions and processes that guide the highly-regulated packaging of DNA into chromatin, as well as chromatin features that act to switch gene expression on and off. Basic research has enabled chromatin engineering by rational design for building new tools to further understand chromatin, and for applications such as molecular interventions of cellular disease states. This review highlights key discoveries in chromatin research and engineering efforts that have been supported by this knowledge.

Rene Daer to present at SEED 2017

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Congratulations to Haynes lab PhD student Rene Daer, who has been invited to give a poster presentation at the 2017 Synthetic Biology: Engineering, Evolution & Design (SEED) conference in Vancouver, Canada May 21-21, 2016. She will present her latest work on manipulating chromatin in human cells to enhance CRISPR efficiency. Rene is a fifth-year graduate student in the Biological Design program.

Research – bioRxiv Pre-print – Tandem histone binding domains enhance the activity of a synthetic chromatin effector

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Tandem histone-binding domains enhance the activity of a synthetic chromatin effector
Tekel SJ, Vargas DA, Song L, LaBaer J, Haynes KA. (2017) bioRxiv. http://biorxiv.org/content/early/2017/06/03/145730

Here, we report the behavior of a re-engineered PcTF,  a gene-regulating fusion protein that is designed to activate genes that have been suppressed by chromatin condensation in cancer cells. We added an extra histone-binding domain to create Pc2TF and observed 2- to 4-fold enhancement of target binding and target gene activation. The new design was inspired by natural proteins that also have double-motifs that contribute to target affinity. The specific combination of motifs in Pc2TF does not exist in nature. By using design rules inferred from pre-existing motif patterns, we have improved the performance a novel synthetic chromatin effector. This improved activity advances PcTF towards clinical translation for anti-cancer therapy.

Related resources:

  1. In Vitro Development of Synthetic Chromatin Proteins That Function in Live Cells. FASEB. Abstract. http://www.fasebj.org/content/31/1_Supplement/922.8.short