A sensitive switch for visualizing natural gene silencing in single cells
Haynes KA, Ceroni F, Flicker D, Younger A, Silver PA. (2012) ACS Synth. Biol. 1: 99–106. PMID: 22530199
We designed a synthetic gene switch that expressed cyan fluorescent protein in the presence of microRNAs, which are biomarkers for cell development and disease. The switch was designed to be sensitive to small, hard-to-detect microRNAs in live cells. Here, we demonstrated that the switch responded to natural as well as artificial proof-of-concept microRNA signals.