Research – bioRxiv Pre-print – Activation of tumor suppressor genes in breast cancer cells by a synthetic chromatin effector
Activation of tumor suppressor genes in breast cancer cells by a synthetic chromatin effector
Olney KC, Nyer DB, Wilson Sayres MA, Haynes KA. (2017) bioRxiv. http://www.biorxiv.org/content/early/2017/09/07/186056
Certain types of breast cancer can be difficult to treat because breast cancer cells in different patients are not completely identical. Here, we measured the expression levels of genes in drug responsive and non-responsive (triple-negative) lab-grown breast cancer cells. In agreement with findings from other research groups, we observed that certain groups of genes are commonly or differentially expressed. Importantly, a large group of genes is silenced in breast cancer cells compared to less cancerous cells. In cancers, certain overactive proteins silence genes by inducing tight chromatin packing. We used a synthetic fusion protein called PcTF to bind and disrupt cancer-associated gene silencing. Dozens of genes, including fifteen different anti-cancer genes became activated in all of the cancer cell types, including the triple-negative cells. PcTF has the potential to act as a powerful therapeutic protein (biologic) that activates multiple anti-cancer genes at once.