Open Science

Recently Shared DNA Plasmids

PcTF (hPCD-TF) in MV2 | Benchling | DNASU
Synthetic Polycomb-based transcription factor to activate epigenetically-silenced genes.

TF in MV2 | Benchling | DNASU
N-terminal-truncated variant of PcTF for control experiments.

hPCD-RFP in MV2 | Benchling | DNASU
N-terminal-truncated variant of PcTF for control experiments.

VP64 in MV14 | Benchling | DNASU
VP64 transcription activator cloned in-frame with, and at the C-terminus of, a Gal4 DNA binding domain and mCherry (RFP) from the MV14 vector.

RELA (Homo sapiens p65) in MV14 | Benchling | DNASU
p65 transcription activator cloned in-frame with, and at the C-terminus of, a Gal4 DNA binding domain and mCherry (RFP) from the MV14 vector.

Annotated Sequence Collections

Collection Pages DNASU Contributor(s) Publication(s)
Chromatin CRISPR Interference Benchling UnSC00746685
UnSC00746686
Rene Daer Daer 2016
Gal4 DNA-binding Fusion Transcription Regulators Benchling HsCD00812387
HaCD00812388
Cassandra Barrett
Histone-binding Fusion Transcription Factors Benchling ReCD00812741
ReCD00812742
ReCD00812743
Karmella Haynes Haynes 2011
Nyer 2017
Quorum Sensing Collection Benchling
iGEM
Brady Dennison
Ernesto Luna
Jiaqi Wu
Jimmy Xu

Deep Sequencing Data

Transcriptome-wide response to synthetic chromatin protein PcTF.
Contributors: Haynes KA
Publication: Nyer DB te al. (2017) Regulation of cancer epigenomes with a histone-binding synthetic transcription factor. Nature Genomic Medicine.
Gene Expression Omnibus (GEO): Accession Number GSE94120

Transcriptome-wide response to synthetic chromatin protein PcTF.
Contributors: Haynes KA, Wilson Sayres MA, Olney KC, Nyer DB
Publication: Olney KC te al. (2017) Activation of tumor suppressor genes in breast cancer cells by a synthetic chromatin effector. bioRxiv https://www.biorxiv.org/content/early/2017/09/07/186056.
Gene Expression Omnibus (GEO): Accession Number GSE103520

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